AcroScreen - Photometric Enzyme Method for Detecting Acrosin Activity in Spermatozoa
AcroScreen
Description
Photometric Enzyme Method for Detecting Acrosin Activity in Spermatozoa
Features
- 4-component kit
- 1-step 90-minute incubation
- colorimetric result
- protease control included
- 96 determinations per kit
- 18-month shelf life
Background
Acrosin is a sperm acrosomal protease that has an essential role in the fertilization process. Acrosin is released during the acrosome reaction. It has been shown to be involved in the subsequent binding of spermatozoa to the egg's zona pellucida and/or the penetration of spermatozoa through the zona pellucida. Low levels of acrosin appear to be associated with subfertility and infertility. Studies have further shown that total acrosin activity positively correlates with in vitro fertilization rates and a low acrosin activity in an otherwise normal ejaculate is associated with an impaired hamster egg penetration.
The acrosin activity of human ejaculates varies independently of the standard semen parameters such as sperm concentration, percent motility, sperm motion characteristics, and morphology. Thus, the enzyme may be an additional marker for human semen quality.
Manufactured by Scopescreen, formerly BioScreen.
Products available from Bioscreen Inc. for evaulating male infertility:
- FructoScreen® – measures fructose in seminal plasma
- ImmunoSpheres® Anti-IgA – detects IgA antisperm antibodies
- ImmunoSpheres® Anti-IgG – detects IgG antisperm antibodies
- ImmunoSpheres® Anti-IgM – detects IgM antisperm antibodies
- ImmunoSpheres® Anti Ig(H&L) – detects Ig(H&L) antisperm antibodies
- LeucoScreen™ – detects peroxidase-positive cells in semen; now includes a Peroxidase control
- MarScreen® IgA – detects IgA antisperm antibodies
- MarScreen® IgG – detects IgG antisperm antibodies
- MarScreen® IgM – detects IgM antisperm antibodies
- Sperm Antibody (IgG) Controls serum for antibody testing
- Sperm Antibody (IgA/IgG) Controls serum for antibody testing
